Chipinda Chachikhalidwe

Mbale chikhalidwe chikhalidwe akhoza kugawidwa pansi lathyathyathya ndi kuzungulira pansi (U-mphako ndi V woboola pakati) malinga ndi mawonekedwe a pansi; zitsime zachikhalidwe ndi 6, 12, 24, 48, 96, 384, 1536, ndi zina .; kutengera izi, pali mbale ya Terasaki komanso mbale wamba yamaselo. Kusankhidwa kwake kumatengera mtundu wamaselo otukuka, kuchuluka kwa chikhalidwe ndi zoyeserera zosiyanasiyana.

(1) Kusiyana ndi kusankha kwa lathyathyathya-pansi ndi kozungulira-bottomed (U woboola pakati ndi V woboola pakati) mbale mbale

Mitundu yosiyanasiyana yama mbale azikhalidwe imagwiritsidwa ntchito mosiyanasiyana. Pogwiritsa ntchito maselo, pansi pake pamagwiritsidwa ntchito, zomwe zimakhala zosavuta kuziwona pansi pa microscope, zimakhala ndi malo omveka bwino, ndipo kutalika kwa chikhalidwe cha selo kumakhala kosasinthasintha. Chifukwa chake, poyesa zinthu monga MTT, kaya ndi yama cell yolumikizidwa kapena yoyimitsidwa, mbale yonyika pansi imagwiritsidwa ntchito kwambiri. Chidebe chachikhalidwe chokhazikika chiyenera kugwiritsidwa ntchito kuyerekezera mtengo wa mayamwidwe. Samalani kwambiri nkhaniyo. Chizindikiro "Tissue Culture (TC) Chochitidwa" ndichachikhalidwe cha cell. Ma mbale opangidwa ngati U kapena V amagwiritsidwa ntchito pakakhala zofunikira zina. Mwachitsanzo, mu immunology, pomwe ma lymphocyte awiri ndi chikhalidwe chosakanikirana, awiriwo amafunika kulumikizana kuti alimbikitse. Poterepa, mbale zopangidwa ngati U zimagwiritsidwa ntchito nthawi zambiri chifukwa ma cell amadzera pang'ono chifukwa cha mphamvu yokoka. Chingwe chachikhalidwe chotsika-pansi chidzagwiritsidwanso ntchito poyesa kuphatikiza kwa isotope, ndipo ndikofunikira kugwiritsa ntchito osonkhanitsa maselo kuti atolere maselo achikhalidwe, monga "chikhalidwe chosakanikirana cha lymphocyte" ndi zina zambiri. Ma mbale opangidwa ndi V amagwiritsidwa ntchito kwambiri popha ma cell ndi kuyesa kwa immunological hemagglutination. Kuyesa kwa kupha maselo kumatha kusinthidwa ndi mbale yopangidwa ndi U (mutatha kuwonjezera ma cell, centrifugation mwachangu).

(2) Kusiyana pakati pa mbale ya Terasaki ndi mbale wamba yamaselo

Teraaki mbale imagwiritsidwa ntchito makamaka pofufuza za crystallographic, ndipo kapangidwe kake ndi kothandiza pakuwunika kwa kristalo ndi kapangidwe kake. Pali njira ziwiri zokhalira pansi ndikuponya, ndikuwonekera ndi kapangidwe kazogwiritsira ntchito njira ziwirizi ndizosiyananso. Sankhani polima wa kristalo monga chinthucho, ndipo chinthu chapaderacho ndichabwino pakuwona mawonekedwe a kristalo. Mbale chikhalidwe chikhalidwe makamaka zopangidwa PS zakuthupi, ndi zinthu mankhwalawa sufface, amene ali yabwino kukula selo ndi kukula. Zachidziwikire, pali zinthu zokula zama cell planktonic, komanso malo omangika otsika.

(3) Kusiyana pakati pa mbale yachikhalidwe ndi microplate

Ma mbale okhala ndi ma enzyme amakhala okwera mtengo kwambiri kuposa mbale zama cell. Ma cell a cell amagwiritsidwa ntchito kwambiri pakapangidwe kamaselo ndipo amathanso kugwiritsidwa ntchito poyesa kuchuluka kwa mapuloteni. Mbale zolembedwa ndi ma enzyme zimaphatikizapo mbale zokutira ndi mbale zoyankhira. Nthawi zambiri, safunikira kuti azigwiritsidwa ntchito pachikhalidwe cha khungu. Amagwiritsidwa ntchito makamaka pochita immunoenzyme reaction. Kuzindikira mapuloteni pambuyo pake kumafunikira zofunikira kwambiri komanso madzi amtundu wina omwe amadziwika kuti ndi enzyme.

(4) Pansi pazitsime zamipanda yazikhalidwe zosiyanasiyana komanso kuchuluka kwa madzi sayenera kukhala akuya, makamaka pakati pa 2 ~ 3mm, ndipo pansi pazitsime zosiyanasiyana zitha kugwiritsidwa ntchito kuwerengera chitsime chilichonse. Kuchuluka kwa madzi oti awonjezeke (onaninso tebulo ili m'munsiyi). Ngati kuchuluka kwa madzi omwe awonjezeredwa ndi ochulukirapo, zimakhudza kusinthana kwa gasi (oxygen), ndipo ndikosavuta kusefukira ndikupangitsa kuipitsa panthawi yosuntha. Kuchuluka kwa ma cell omwe akuwonjezeredwa kumadalira cholinga cha kuyeserera kosavuta.